Hirschsprung′s disease diagnosis: Comparison of immunohistochemical, hematoxilin and eosin staining

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Hirschsprung's disease diagnosis: Comparison of immunohistochemical, hematoxilin and eosin staining

BACKGROUND The diagnosis of Hirschsprung's disease (HD) is based on the absence of ganglion cells. In hemotoxilin and eosin (H and E) as well as acetylcholine esterase staining there are limitations in the diagnosis of immature ganglion cells in neonates. METHODS In this prospective study, 54 biopsies taken from suspected HD patients (five mucosal specimens and 49 full thickness specimens) we...

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Is hematoxylin-eosin staining in rectal mucosal and submucosal biopsies still useful for the diagnosis of Hirschsprung disease?

BACKGROUND Hematoxylin-eosin (HE) staining of a full-thickness rectal wall fragment is classically used for the diagnosis of Hirschsprung disease (HD). However, this technique requires large fragments for a better diagnosis. Additionally, the histochemical and immunohistochemical methods of staining small fragments of rectal mucosal and submucosal biopsies are not available in all centers. Ther...

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Hirschsprungs disease with congenital hypothyroidism.

We report a female newborn baby who presented with vomiting and abdominal distension on day 21 of life. Examination revealed facial puffiness, open posterior fontanelles, dry skin, cold peripheries and prominent abdominal veins with visible peristalsis. Barium enema revealed dilated proximal colon, empty rectum, funnel like transition zone between proximal dilated and distal constricted bowel. ...

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The diagnosis of Wesselsbron disease in a new-born lamb by immunohistochemical staining of viral antigen.

Wesselsbron disease (WSL) was diagnosed in a 2-d-old lamb on a farm in the north-eastern Free State Province where a few abortions and neonatal deaths occurred in sheep in April 1994. The liver of the lamb was slightly swollen and orange-brown and, microscopically, it revealed single or small groups of necrotic hepatocytes that were randomly scattered throughout the lobules. Other histopatholog...

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ژورنال

عنوان ژورنال: Journal of Indian Association of Pediatric Surgeons

سال: 2009

ISSN: 0971-9261

DOI: 10.4103/0971-9261.55153